Evaluation of an electronic air filter for filtrating bacteria and viruses from indoor air.

This study compared the filtrating efficiency (FE) of a commercial electronic air filter for filtering bacteria and viruses from contaminated air with a high efficiency particulate air (HEPA) filter. An enclosed chamber was constructed, in the middle of which an air filter was placed for testing. MTB H37Ra and T7 virus at concentrations of 5x10(8) each were sprayed into one side of the chamber using a nebulizer and the sprayed samples were collected by an impinger air-sampler on the other side. MTB and T7 viruses were detected by PCR and culture. The PCR could detect samples down to 10 fg for MTB H37Ra and 1 pg for T7 virus. Most MTB H37Ra sprayed failed to culture. S. aureus at a concentration of 10(5) cfu and E. coli at a concentration of 10(4) cfu along with T7 virus were filtered out with a FE of more than 99%. T7 virus has a particle size of 0.04 microm, S. aureus has a particle size of 1 microm and E. coli has a particle size of 2 microm.

The first isolation of ustilaginomycetous anamorphic yeasts, Pseudozyma species, from patients' blood and a description of two new species: P. parantarctica and P. thailandica.

The genus Pseudozyma is ustilaginomycetous anamorphic yeasts, and are mainly isolated from plants. We isolated three Pseudozyma strains from the blood of patients in Thailand. While one isolate was identified as P. antarctica by rDNA sequence analysis, the other two were considered to be new species and were named P. parantarctica and P. thailandica. The three isolates proved to be resistant to 5-flucytosine, and P. thailandica was also resistant to fluconazole and itraconazole. As far as we know, this is the first isolation of Pseudozyma strains from humans. The two new species are described.